ISOLATION OF NUCLEI FROM YEAST
نویسندگان
چکیده
منابع مشابه
Isolation of Nuclei from Yeast
A method for isolation of nuclei from Saccharomyces cervisiae in high yield is described. The DNA/protein ratio of the isolated nuclei is 10 times higher than that of whole cells. Examination of these nuclei in phase and electron microscopes has shown them to be round bodies having a double membrane, microtubules, and a dark crescent at one end. The optimum conditions for extraction and resolut...
متن کاملIsolation of nuclei for chromatin analysis in fission yeast.
The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes <25 min and is suitable for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by immunopreci...
متن کاملNuclei Isolation from Nematode Ascaris.
Preparing nuclei is necessary in a variety of experimental paradigms to study nuclear processes. In this protocol, we describe a method for rapid preparation of large number of relatively pure nuclei from Ascaris embryos or tissues that are ready to be used for further experiments such as chromatin isolation and ChIP-seq, nuclear RNA analyses, or preparation of nuclear extracts (Kang et al., 20...
متن کاملThe isolation of globulins from cellular nuclei.
Behrens in 1932 (1) developed a method for isolating cellular nuclei. Lyophilized tissue was finely ground and the nuclei were isolated from other cellular debris by repeated centrifugation in organic solvents of suitable densities. The greater density of the nuclei makes this procedure possible. The method has been employed by various workers (2-5). Dounce et al. (4) reported that approximatel...
متن کاملIsolation of nuclei from Aspergillus nidulans protoplasts.
Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifugation was applied. The resulting nuclei were of good purity and morphological s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1971
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.49.2.423